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Tissue Preparation and Immunostaining of Mouse Craniofacial Tissues and Undecalcified Bone
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Developmental Biology
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JoVE Journal Developmental Biology
Tissue Preparation and Immunostaining of Mouse Craniofacial Tissues and Undecalcified Bone

Tissue Preparation and Immunostaining of Mouse Craniofacial Tissues and Undecalcified Bone

DOI:
10.3791/59113-v

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10:03 min

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May 10, 2019

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, ,
1The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory for Oral Biomedicine of Ministry of Education, School and Hospital of Stomatology,Wuhan University, 2Department of Biologic and Materials Sciences, School of Dentistry,University of Michigan

Chapters

  • 00:04Title
  • 01:13Preparation of Embryonic Tissues
  • 03:38Preparation of Postnatal Undecalcified Hard Tissues
  • 04:15Cryosectioning
  • 05:18Immunofluorescence Staining
  • 06:50Results: Immunostained Mouse Craniofacial Tissues and Undecalcified Bone
  • 09:03Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we present a detailed protocol to detect and quantify protein levels during craniofacial morphogenesis/pathogenesis by immunostaining using mouse craniofacial tissues as examples. In addition, we describe a method for preparation and cryosectioning of undecalcified hard tissues from young mice for immunostaining.

Tags

ImmunofluorescenceImmunostainingUndecalcified BoneAntigen RetrievalDecalcificationCryoprotectionEmbeddingFreezingSectioningPBSPFAEDTASucroseOCT Compound

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