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Enhanced Yeast One-hybrid Screens To Identify Transcription Factor Binding To Human DNA Sequences
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JoVE Journal Genetics
Enhanced Yeast One-hybrid Screens To Identify Transcription Factor Binding To Human DNA Sequences

Enhanced Yeast One-hybrid Screens To Identify Transcription Factor Binding To Human DNA Sequences

DOI:
10.3791/59192-v

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11:25 min

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February 11, 2019

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, , ,
1Department of Biology,Boston University, 2Bioinformatics Program,Boston University

Chapters

  • 00:04Title
  • 00:49Spotting a Transcription Factor (TF) Array
  • 04:19Enhanced Yeast One-hybrid (eY1H) Screen
  • 08:48Results: Examples of eY1H Readout Plates and Potential Problems
  • 10:28Conclusion

Summary

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Here, we present an enhanced yeast one-hybrid screening protocol to identify the transcription factors (TFs) that can bind to a human DNA region of interest. This method uses a high-throughput screening pipeline that can interrogate the binding of >1,000 TFs in a single experiment.

Tags

Transcription FactorYeast One-hybridDNA SequenceFunctional GenomicsGene PromotersEnhancersNon-coding VariantsArray RobotHigh Density96-well Plates384-well Plates1536-well PlatesColony ArraySc Minus Tryptophan

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