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Modeling Breast Cancer via an Intraductal Injection of Cre-expressing Adenovirus into the Mouse Mammary Gland
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Cancer Research
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JoVE Journal Cancer Research
Modeling Breast Cancer via an Intraductal Injection of Cre-expressing Adenovirus into the Mouse Mammary Gland

Modeling Breast Cancer via an Intraductal Injection of Cre-expressing Adenovirus into the Mouse Mammary Gland

DOI:
10.3791/59502-v

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06:29 min

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June 07, 2019

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, ,
1Division of Genetics, Department of Medicine,Brigham and Women’s Hospital, 2Department of Medicine,Harvard Medical School

Chapters

  • 00:04Title
  • 00:34Preoperative Preparation
  • 01:48Intraductal Injection
  • 03:53Results: Key Features of Genetic Manipulations of Mammary Epithelial Cells Based on Intraductal Injection of Ad-Cre
  • 05:25Conclusion

Summary

Automatic Translation

Automatic Translation

The goal of this protocol is to describe a new breast cancer modeling approach based on the intraductal injection of Cre-expressing adenovirus into mouse mammary glands. This approach allows both cell-type- and organ-specific manipulation of oncogenic events in a temporally controlled manner.

Tags

Breast CancerMouse ModelIntraductal InjectionCre-expressing AdenovirusMammary GlandMammary Epithelial CellsSurgical ProcedureAnesthesiaAnalgesiaHair RemovalDisinfectionIncisionNipple RemovalHamilton SyringeDyeDuctal Tree

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