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Analysis of Lipid Droplet Content in Fission and Budding Yeasts using Automated Image Processing
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Immunology and Infection
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JoVE Journal Immunology and Infection
Analysis of Lipid Droplet Content in Fission and Budding Yeasts using Automated Image Processing

Analysis of Lipid Droplet Content in Fission and Budding Yeasts using Automated Image Processing

DOI:
10.3791/59889-v

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08:43 min

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July 17, 2019

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, , ,
1Faculty of Science,Charles University

Chapters

  • 00:04Title
  • 00:31Lipid Droplet Staining
  • 02:39Imaging Setup and Acquisition
  • 04:40Image Analysis: Quality Check
  • 05:39Image Analysis: MATLAB
  • 06:27Results: Changes in Lipid Droplet Number, Size, and Lipid Content in Three Morphologically Distinct Yeast Species
  • 08:06Conclusion

Summary

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Here, we present a MATLAB implementation of automated detection and quantitative description of lipid droplets in fluorescence microscopy images of fission and budding yeast cells.

Tags

Lipid DropletYeastFissionBuddingAutomated Image ProcessingMicroscopyCell CultureStainingQuantitative Analysis

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