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Identifying Mutations by High Resolution Melting in a TILLING Population of Rice
JoVE Journal
Genetics
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JoVE Journal Genetics
Identifying Mutations by High Resolution Melting in a TILLING Population of Rice
DOI:

06:10 min

September 02, 2019

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Chapters

  • 00:04Title
  • 00:25DNA Extraction
  • 01:15PCR Amplification
  • 02:34HRM Scanning and Mutation Confirmation
  • 04:29Results: HRM Curves and Mutation
  • 05:41Conclusion

Summary

Automatic Translation

In this article, we present the protocol that is described as high-resolution melting analysis (HRM)-based Target Induced Local Lesions In Genomes (TILLING). This method utilizes fluorescence changes during the melting of the DNA duplex and is suitable for high-throughput screening of both insertion/deletion (Indel) and single base substition (SBS).

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