Journal
/
Immunology and Infection
/
Assessment of Lymphocyte Migration in an Ex Vivo Transmigration System
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
Assessment of Lymphocyte Migration in an Ex Vivo Transmigration System

Assessment of Lymphocyte Migration in an Ex Vivo Transmigration System

DOI:
10.3791/60060-v

•

10:25 min

•

September 20, 2019

•

,
1Department of Internal Medicine,University of Utah, 2Research Service,VA Nebraska Iowa Health Care System, 3Department of Internal Medicine,University of Nebraska Medical Center, 4Department of Environmental, Agricultural & Occupational Health,University of Nebraska Medical Center

Chapters

  • 00:04Title
  • 00:50Isolation of CD4+ T Cells from Spleens and Lungs of OVA-challenged Mice
  • 04:25Ex vivo Transmigration Procedure
  • 06:39Quantification of Ex vivo Transmigration
  • 08:06Results: Assessment of Lymphocyte Migration under Various Conditions
  • 09:42Conclusion

Summary

Automatic Translation

Automatic Translation

In this protocol, lymphocytes are placed in the top chamber of a transmigration system, separated from the bottom chamber by a porous membrane. Chemokine is added to the bottom chamber, which induces active migration along a chemokine gradient. After 48 h, lymphocytes are counted in both chambers to quantitate transmigration.

Tags

Lymphocyte MigrationTransmigrationImmune ResponseChemokinesILC2Ex VivoCell IsolationCell CountingCell Suspension

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

Human T Lymphocyte Isolation, Culture and Analysis of Migration In Vitro

Non-invasive Imaging of Leukocyte Homing and Migration in vivo

Quantitative Assessment of Human Neutrophil Migration Across a Cultured Bladder Epithelium

Assessing the Innate Sensing of HIV-1 Infected CD4+ T Cells by Plasmacytoid Dendritic Cells Using an Ex vivo Co-culture System.

An Ex vivo Model of an Oligodendrocyte-directed T-Cell Attack in Acute Brain Slices

Lymphocyte Isolation from Human Skin for Phenotypic Analysis and Ex Vivo Cell Culture

Isolation and Ex Vivo Culture of Vδ1+CD4+γδ T Cells, an Extrathymic αβT-cell Progenitor

Flow Cytometric Analysis of Particle-bound Bet v 1 Allergen in PM10

Murine Lymphocyte Labeling by 64Cu-Antibody Receptor Targeting for In Vivo Cell Trafficking by PET/CT

Analysis of Lymphocyte Extravasation Using an In Vitro Model of the Human Blood-brain Barrier

Read Article