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Assessment of Lymphocyte Migration in an Ex Vivo Transmigration System
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Assessment of Lymphocyte Migration in an Ex Vivo Transmigration System
DOI:

10:25 min

September 20, 2019

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Chapters

  • 00:04Title
  • 00:50Isolation of CD4+ T Cells from Spleens and Lungs of OVA-challenged Mice
  • 04:25Ex vivo Transmigration Procedure
  • 06:39Quantification of Ex vivo Transmigration
  • 08:06Results: Assessment of Lymphocyte Migration under Various Conditions
  • 09:42Conclusion

Summary

Automatic Translation

In this protocol, lymphocytes are placed in the top chamber of a transmigration system, separated from the bottom chamber by a porous membrane. Chemokine is added to the bottom chamber, which induces active migration along a chemokine gradient. After 48 h, lymphocytes are counted in both chambers to quantitate transmigration.

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