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Characterization of Functionally Associated miRNAs in Glioblastoma and their Engineering into Artificial Clusters for Gene Therapy
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Genetics
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JoVE Journal Genetics
Characterization of Functionally Associated miRNAs in Glioblastoma and their Engineering into Artificial Clusters for Gene Therapy

Characterization of Functionally Associated miRNAs in Glioblastoma and their Engineering into Artificial Clusters for Gene Therapy

DOI:
10.3791/60215-v

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09:40 min

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October 04, 2019

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,
1Department of Neurosurgery,Brigham and Women’s Hospital-Harvard Medical School

Chapters

  • 00:04Title
  • 01:19Induction of Cell Differentiation
  • 02:16Validation of miRNA Clusters by Stress-specific Challenges
  • 03:28Characterization of The Functional Convergence of Clustered miRNAs
  • 05:27Assembly of miRNA Modules into a Polycistronic Transgenic Cluster
  • 07:51Results: Functional miRNA Module and Transgene Processing
  • 08:40Conclusion

Summary

Automatic Translation

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Described here is a protocol for characterizing modules of biologically synergistic miRNAs and their assembly into short transgenes, which allows simultaneous overexpression for gene therapy applications.

Tags

MiRNAMicroRNAGlioblastomaGene TherapyArtificial MiRNA ClustersNeural Stem CellsGBM-34 Glioblastoma Stem-like CellsTemozolomideBioinformaticsGene ExpressionCancerPoly-D-lysineCell CultureIn Vitro

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