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Fibroblast-Derived 3D Matrix System Applicable to Endothelial Tube Formation Assay
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1Medical Oncology Department,Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), CIBERONC, 2Department of Medical Oncology,Hospital Universitario Puerta de Hierro de Majadahonda, 3Instituto de Investigaciones Biomédicas Alberto Sols,Consejo Superior de Investigaciones Científicas – Universidad Autónoma de Madrid, CIBERONC, 4Department of Oncology & Pathology,Karolinska Institutet
Chapters
- 00:04Title
- 01:13Preparation of Solutions and Cell Cultures
- 02:32Fibroblast-derived 3D Matrices
- 04:50Tube Formation Assay
- 05:45Results: Effects of PDGF Treatment on ECM Formation and Endothelial Cell Activation
- 06:42Conclusion
The aim of this method is to obtain fibroblast-derived 3D matrices as a natural scaffold for subsequent cellular assays. Fibroblasts are seeded in a pre-treated culture plate and stimulated with ascorbic acid for matrix generation. Matrices are decellularized and blocked to culture relevant cells (e.g., endothelial cells).
