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Production of Dynein and Kinesin Motor Ensembles on DNA Origami Nanostructures for Single Molecule Observation
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Production of Dynein and Kinesin Motor Ensembles on DNA Origami Nanostructures for Single Molecule Observation
DOI:

08:09 min

October 15, 2019

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Chapters

  • 00:04Title
  • 00:46Galactose-Induced Motor Protein Growth, Expression, and Harvesting
  • 01:39Cell Lysis and Soluble Protein Extraction
  • 02:36IgG Affinity Purification
  • 03:17DNA Oligonucleotide Labeling and TEV Cleavage
  • 04:50Chassis Purification
  • 05:56Results: Representative Motor Ensemble Production
  • 07:26Conclusion

Summary

Automatic Translation

The goal of this protocol is to form ensembles of molecular motors on DNA origami nanostructures and observe the ensemble motility using total internal reflection fluorescence microscopy.

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