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Increasing Durability of Dissociated Neural Cell Cultures Using Biologically Active Coralline Matrix
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Increasing Durability of Dissociated Neural Cell Cultures Using Biologically Active Coralline Matrix
DOI:

09:22 min

June 03, 2020

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Chapters

  • 00:04Introduction
  • 00:46Cleaning the Coral Skeleton Pieces and Glass Coverslips
  • 02:07Preparation of Coral Skeleton Grains
  • 02:52Preparation of Coral Grain‐Coated Dishes or Coverslips
  • 04:40Cultivation of Hippocampal Dissociated Cells on Coral Skeleton Grain‐Coated Glass Coverslips
  • 07:26Results: Coral Skeleton Grains and Hippocampal Neural Cells on the Matrix
  • 08:52Conclusion

Summary

Automatic Translation

Dissociated hippocampal cell culture is a pivotal experimental tool in neuroscience. Neural cell survival and function in culture is enhanced when coralline skeletons are used as matrices, due to their neuroprotective and neuromodulative roles. Hence, neural cells grown on coralline matrix show higher durability, and thereby are more adequate for culturing.

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