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Skeletal Phenotype Analysis of a Conditional Stat3 Deletion Mouse Model
JoVE Journal
Biology
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JoVE Journal Biology
Skeletal Phenotype Analysis of a Conditional Stat3 Deletion Mouse Model
DOI:

08:42 min

July 03, 2020

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Chapters

  • 00:04Introduction
  • 00:54Specimen Collection
  • 02:13Paraffin Section Preparation
  • 03:34TRAP Staining
  • 05:34Calcein and Alizarin Red Double Labeling
  • 06:55Results: Effects of Stat3 Deletion on Osteoclast Differentiation
  • 08:09Conclusion

Summary

Automatic Translation

This protocol describes a canonical method to understand the critical genes controlling osteoclast activity in vivo. This method uses a transgenic mouse model and some canonical techniques to analyze skeletal phenotype.

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