Journal
/
Biology
/
Skeletal Phenotype Analysis of a Conditional Stat3 Deletion Mouse Model
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Skeletal Phenotype Analysis of a Conditional Stat3 Deletion Mouse Model

Skeletal Phenotype Analysis of a Conditional Stat3 Deletion Mouse Model

DOI:
10.3791/61390-v

•

08:42 min

•

July 03, 2020

•

, , , , , , ,
1Center of Craniofacial Orthodontics, Department of Oral and Cranio-maxillofacial Science, Ninth People’s Hospital, Shanghai Jiaotong University School of Medicine, Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology,National Clinical Research center of Stomatology, 2Department of Stomatology,Dalian Medical University, 3The 2nd Dental Center, Ninth People’s Hospital, Shanghai Jiaotong University School of Medicine, Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology,National Clinical Research center of Stomatology

Chapters

  • 00:04Introduction
  • 00:54Specimen Collection
  • 02:13Paraffin Section Preparation
  • 03:34TRAP Staining
  • 05:34Calcein and Alizarin Red Double Labeling
  • 06:55Results: Effects of Stat3 Deletion on Osteoclast Differentiation
  • 08:09Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes a canonical method to understand the critical genes controlling osteoclast activity in vivo. This method uses a transgenic mouse model and some canonical techniques to analyze skeletal phenotype.

Tags

Skeletal Phenotype AnalysisGenetically Engineered Mouse ModelHind LimbParaffin SectioningMicro-CT ScanningCalcein And Alizarin Red Double LabelingDecalcificationParaffin Embedding

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

A Neuronal and Astrocyte Co-Culture Assay for High Content Analysis of Neurotoxicity

Analysis of the Development of a Morphological Phenotype as a Function of Protein Concentration in Budding Yeast

In vivo Imaging and Therapeutic Treatments in an Orthotopic Mouse Model of Ovarian Cancer

Analysis of Skeletal Muscle Defects in Larval Zebrafish by Birefringence and Touch-evoke Escape Response Assays

Alternative Cultures for Human Pluripotent Stem Cell Production, Maintenance, and Genetic Analysis

Detection and Analysis of DNA Damage in Mouse Skeletal Muscle In Situ Using the TUNEL Method

Cryosectioning of Contiguous Regions of a Single Mouse Skeletal Muscle for Gene Expression and Histological Analyses

Trans-inner Cell Mass Injection of Embryonic Stem Cells Leads to Higher Chimerism Rates

A Mouse Model of Intestinal Partial Obstruction

Mass Spectrometry Analysis to Identify Ubiquitylation of EYFP-tagged CENP-A (EYFP-CENP-A)

Read Article