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Patch Clamp Recording of Ion Channels Expressed in Xenopus Oocytes
JoVE Journal
Biology
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JoVE Journal Biology
Patch Clamp Recording of Ion Channels Expressed in Xenopus Oocytes
DOI:

08:00 min

October 16, 2008

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Chapters

  • 00:00Title
  • 00:49Introduction
  • 01:35Removing the Vitelline Membrane
  • 03:33Gigaseal Formation
  • 06:05Outside-Out Topology
  • 06:59Conclusion

Summary

Automatic Translation

This is intended as an introduction to patch clamp recording from Xenopus laevis oocytes. It covers vitelline membrane removal, formation of a gigaohm seal (gigaseal), and the optional conversion of the patch to the outside-out topology.

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