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作为一种工具来隔离核糖体的束缚多肽SECM逮捕序列

Article DOI: 10.3791/4027-v • 09:42 min • June 19th, 2012
June 19th, 2012 •
1, 1
1Center for Gene Regulation in Health and Disease, Department of Biological, Geological and Environmental Sciences, Cleveland State University

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我们在这里描述，现在经常使用的孤立稳定结合的核糖体新生肽链复合物（RNC）中的技术。这种技术发现了17个氨基酸长SECM“逮捕序列”可以停止在原核翻译延伸的优势（

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分子生物学，64期，核糖体，新生多肽，合作翻译的蛋白质折叠，平移逮捕，
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