JoVE Journal
Developmental Biology
Developmental Biology
A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
Summary
Complexity of in vivo systems makes it difficult to distinguish between the activation and inhibition of Notch receptor by trans- and cis-ligands, respectively. Here, we present a protocol based on in vitro cell-aggregation assays for qualitative and semi-quantitative evaluation of the binding of Drosophila Notch to trans-ligands vs cis-ligands.