JoVE Journal
Immunology and Infection
Immunology and Infection
A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
Summary
We describe a platform that utilizes a library of isogenic antibiotic resistant Escherichia coli for the dereplication of antibiotics. The identity of an antibiotic produced by bacteria or fungi can be deduced by the growth of E. coli expressing its respective resistance gene. This platform is economically effective and time-efficient.