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15.14: Secuenciación de Maxam-Gilbert

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Molecular Biology

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Maxam-Gilbert Sequencing

15.14: Maxam-Gilbert Sequencing

In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.

Challenges of the Maxam-Gilbert Method

The Maxam-Gilbert Method can only be used to typically resolve up to 400bp in a single run which is comparatively lower than other traditional sequencing methods such as Sanger sequencing. Additionally, the requirement of relatively large amounts of template DNA, coupled with the use of radioisotopes and hazardous chemicals, makes the Maxam-Gilbert method less favorable for routine use. However, because of the direct use of purified DNA, the Maxam-Gilbert method is more accurate than Sanger sequencing.

Present Day Applications of Maxam-Gilbert method

Although the Maxam-Gilbert method is less widely used, it is still preferred for use in some techniques, such as DNA fingerprinting and DNA structural studies.

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