JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Biology
/
涂层生长因子-珠放在背的前脑植
JoVE Journal
Biology
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Biology
Growth Factor-Coated Bead Placement on Dorsal Forebrain Explants
Please note that all translations are automatically generated.
Click here for the English version.
涂层生长因子-珠放在背的前脑植
DOI:
10.3791/134-v
•
15:07 min
•
January 30, 2007
•
D. Spencer Currle
,
Aaron Kolski-Andreaco
,
Edwin S. Monuki
1
Department of Developmental and Cell Biology
,
University of California, Irvine (UCI)
,
2
Department of Physiology and Biophysics
,
University of California, Irvine (UCI)
,
3
Department of Pathology
,
University of California, Irvine (UCI)
Chapters
00:45
Types of beads: Affi-Gel and Heparin Acrylic
01:13
Affi-Gel bead placement
01:18
Preparation of glass capillaries
03:47
Preparation of Affi-Gel beads
09:32
Affi-Gel bead placement on explant
11:27
Preparation of acrylic beads
13:31
Acrylic bead placement on explant
14:32
Example of obtained results
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
该视频演示两种方法准备,并把珠,与生长因子,对发展中国家的大脑皮层的外植体,已被涂。这些珠子可用于诱导前脑植等神经组织的发展空间限制的基因表达。方法是使用阿菲 - 凝胶珠和肝素acryllic珠。
Tags
Growth Factor-coated Bead Placement
Dorsal Forebrain
Explants
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
鼠标背的前脑植隔离
优化的纤维蛋白凝胶珠检测血管生成的研究
配售早期鸡胚生长因子包被的磁珠
大鼠晶状体上皮细胞外植体的制备及文化学习终末分化
分离纯化
果蝇
外周神经元
从外植体生长的首要人权支气管上皮细胞
递延生长抑制试验来量化竞争性细菌来源的抗菌剂的影响
用数据无关采集质谱法定量测定点特异蛋白赖氨酸乙酰化和琥珀酰化化学计量学
中通量筛选法评估对精子中 ca
2 +
信号和反应的影响
用于下一代测序的基于磁珠的蚊子DNA提取协议
Read Article