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涂层生长因子-珠放在背的前脑植
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JoVE Journal Biology
Growth Factor-Coated Bead Placement on Dorsal Forebrain Explants
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涂层生长因子-珠放在背的前脑植

DOI:
10.3791/134-v

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15:07 min

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January 30, 2007

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, ,
1Department of Developmental and Cell Biology,University of California, Irvine (UCI), 2Department of Physiology and Biophysics,University of California, Irvine (UCI), 3Department of Pathology,University of California, Irvine (UCI)

Chapters

  • 00:45Types of beads: Affi-Gel and Heparin Acrylic
  • 01:13Affi-Gel bead placement
  • 01:18Preparation of glass capillaries
  • 03:47Preparation of Affi-Gel beads
  • 09:32Affi-Gel bead placement on explant
  • 11:27Preparation of acrylic beads
  • 13:31Acrylic bead placement on explant
  • 14:32Example of obtained results

Summary

Automatic Translation

Automatic Translation

该视频演示两种方法准备，并把珠，与生长因子，对发展中国家的大脑皮层的外植体，已被涂。这些珠子可用于诱导前脑植等神经组织的发展空间限制的基因表达。方法是使用阿菲 - 凝胶珠和肝素acryllic珠。

Tags

Growth Factor-coated Bead PlacementDorsal ForebrainExplants

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