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从酵母细胞线粒体的纯化
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JoVE Journal
Biology
Purification of Mitochondria from Yeast Cells
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从酵母细胞线粒体的纯化
DOI:
10.3791/1417-v
•
10:39 min
•
August 24, 2009
•
Christopher Gregg
,
Pavlo Kyryakov
,
Vladimir I. Titorenko
1
Department of Biology
,
Concordia University
Chapters
00:00
Title
01:05
Introduction
01:37
Harvesting Yeast Cells
03:55
Isolating the Crude Mitochondrial Fraction
06:44
Purifying the Mitochondria
08:59
Results of Purifying Mitochondria by Sucrose Density Centrifugation
09:59
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们描述了一个快速和有效的方法纯化的酵母线粒体<em>酿酒酵母</em>。这种方法使高产纯线粒体隔离,其他细胞器的污染基本上是免费的,并保留其净化后的结构和功能完整性。
Tags
Purification
Mitochondria
Yeast Cells
ATP Production
Aerobic Metabolism
Eukaryotic Cells
Organelles
Apoptotic Cell Death
Cell Survival
Mammalian Development
Neuronal Development
Intracellular Signaling
Longevity Regulation
Saccharomyces Cerevisiae
Model Organism
Mitochondrial Isolation
Purity
Contamination-free
Structural Integrity
Functional Integrity
Essential Mitochondrial Processes
Mitochondrial Structure And Function Analysis
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