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组织有针对性的胚胎嵌合体:斑马鱼原肠胚细胞移植
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Tissue Targeted Embryonic Chimeras: Zebrafish Gastrula Cell Transplantation
Please note that all translations are automatically generated.
Click here for the English version.
组织有针对性的胚胎嵌合体:斑马鱼原肠胚细胞移植
DOI:
10.3791/1422-v
•
18:57 min
•
September 11, 2009
•
Elizabeth R. Deschene
,
Michael J. Barresi
1
Department of Biological Sciences
,
Smith College
Chapters
00:00
Title
01:22
Introduction
02:01
Preparing the Microinjection Plate and Needles
06:03
Microinjection of Fluorescent Alexa Fluor® 594 Dextran
07:03
Embryo and Transplant Apparatus Setup
10:01
Gastrula Stage Cell Transplants
13:26
Visualizing and Imaging Embryos
15:31
Gastrula Cell Transplantation Results
18:08
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
斑马鱼细胞移植使遗传学和胚胎学的结合,产生组织特异性嵌合体。这个视频演示胚囊上演细胞移植,允许我们的实验室,以调查在合缝形成前脑星形胶质细胞的人口和具体的指导线索的角色。
Tags
Zebrafish Gastrula
Cell Transplantation
Developmental Biology
Cell Functions
Protein Misexpression
Forebrain Commissures
Neuron-glial Cell Interactions
Astroglial Populations
Guidance Cues
Projecting Axons
Midline Crossing
Zebrafish Embryos
Ectopic Cell Placement
Gene Misexpression
Vital Dye Tracer
Fluorescent Protein Expression
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