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Chapters
- 00:05Title
- 00:41Introduction
- 01:03Extraction of Total RNA using TRIzol Reagent
- 03:55RNA Cleanup using the Qiagen RNEasy Mini Kit
- 07:57cDNA Synthesis using the Invitrogen SuperScript First-Strand System
- 10:28cDNA Isolation and Precipitation
- 13:50Representative Results/Outcome
- 14:57Conclusion
Die Isolierung von hoher Qualität, ist intakte RNA ein wesentlicher Schritt in vielen Labor-Protokolle. Hier zeigen wir, die RNA-Extraktion aus ganzen Zebrafisch-Embryos und cDNA-Synthese für die spätere Anwendung in verschiedenen experimentellen Verfahren, einschließlich Genexpression Microarray-Analyse.
Tags
RNA IsolationEmbryonic ZebrafishCDNA SynthesisGene Expression AnalysisHigh Quality RNAIntact RNADegraded SampleImpuritiesSolid TechniquesSafeguardsQuality Control ChecksSuperior SampleCommercial RNA Isolation KitChemical DenaturantDNA RemovalRNA StabilityCDNA Synthesis KitCleavage By RNAsesGene Expression AssayStable CDNA ProductMicroarray AnalysisRT-PCRLong-term Storage
