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脂肪酸の定量的なPhosphoproteomicsは刺激を受けて
サッカロマイセスセレビシエ</em
JoVE Journal
Biology
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JoVE Journal
Biology
Quantitative Phosphoproteomics in Fatty Acid Stimulated
Saccharomyces cerevisiae
Please note that all translations are automatically generated.
Click here for the English version.
脂肪酸の定量的なPhosphoproteomicsは刺激を受けて
サッカロマイセスセレビシエ</em
DOI:
10.3791/1474-v
•
15:41 min
•
October 12, 2009
•
Ramsey A. Saleem
,
John D. Aitchison
1
Institute for Systems Biology
Chapters
00:00
Title
00:44
Introduction
01:02
Growing Isotopically Light and Isotopically Heavy Yeast Cells
03:06
Lysing Cells
06:35
Isolation and Fractionation of Peptides
06:38
Alkylation and Reduction
10:32
Desalting
11:54
Peptide Fractionation
13:11
Enrichment of Phosphopeptides
14:51
Representative Results
15:14
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
冷凍融解、尿素solubilziation、HILIC分画とリン酸化ペプチドのIMAC濃縮を用いた定量的リン酸化の手順の説明。
Tags
Quantitative Phosphoproteomics
Fatty Acid Stimulation
Saccharomyces Cerevisiae
Growth And Stimulation
Oleate
Isotopically Heavy And Light Cells
Cryolysis Procedure
Ball Mill Grinder
Urea Solubilization
Metabolically Inactive State
Phosphorylation Preservation
Preventing Reorientation
Cell Lysis
Reduction
Alkylation
Trypsin Digestion
Desalting On C18 Columns
Sample Complexity Reduction
Fractionation Using Hydrophilic Interaction Chromatography (HILIC)
Phosphopeptides Elution Profile
Immobilized Metal Chromatography
Charge-based Affinities
Mass Spectrometry Analysis
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