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蛍光顕微鏡による可視化単分子のDNAの複製
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Visualizing Single-molecule DNA Replication with Fluorescence Microscopy
Please note that all translations are automatically generated.
Click here for the English version.
蛍光顕微鏡による可視化単分子のDNAの複製
DOI:
10.3791/1529-v
•
15:57 min
•
October 09, 2009
•
Nathan A. Tanner
,
Joseph J. Loparo
,
Antoine M. van Oijen
1
Department of Biological Chemistry and Molecular Pharmacology
,
Harvard Medical School
Chapters
00:00
Title
00:42
Introduction
01:11
Preparing the DNA Replication Template
02:44
Preparing Functionalized Coverslips
05:46
Assembling the Flow Chamber
10:03
Single-Molecule Replication Experiment
13:18
Single Molecule DNA Replication Data Analysis and Representative Results
15:00
Conclusion
Summary
Automatic Translation
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Automatic Translation
このプロトコルは、リアルタイムで個々のreplisomesによってDNA複製を視覚化するためのシンプルな単一分子蛍光顕微鏡の技法を示しています。
Tags
Single-molecule DNA Replication
Fluorescence Microscopy
Real-time Method
Circular DNA Template
Replication Proteins
Nucleotides
Growing DNA End
Replication Rate
Completed DNA Products
Processivity Of Replication
Intercalating Dye
Fluorescence Microscope
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