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Journal
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Biology
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大会，加载和分析超速离心机样品池对齐
JoVE Journal
Biology
Author Produced
This content is Free Access.
JoVE Journal
Biology
Assembly, Loading, and Alignment of an Analytical Ultracentrifuge Sample Cell
Please note that all translations are automatically generated.
Click here for the English version.
大会，加载和分析超速离心机样品池对齐
DOI:
10.3791/1530-v
•
11:36 min
•
November 05, 2009
•
Andrea Balbo
,
Huaying Zhao
,
Patrick H. Brown
,
Peter Schuck
1
National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health
,
Dynamics of Macromolecular Assembly, Laboratory of Bioengineering and Physical Science
Chapters
00:00
Introduciton
00:21
Materials
02:21
Sample Cell Assembly and Torque
07:07
Loading and Sealing the Sample Cell
09:23
Counterbalance and Sample Cell Alignment
Summary
Automatic Translation
English (Original)
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Automatic Translation
分析超速离心机（AUC）的样品池持有样品和参考缓冲器，并在实验过程中，暴露在高真空和转子速度高达60,000 RPM。此影片将展示装配，装载和对准这非常重要的组成部分的AUC实验所必需的细节一丝不苟。
Tags
Analytical Ultracentrifuge
AUC
Sedimentation Velocity
Sedimentation Equilibrium
Protein Analysis
Sample Cell Assembly
Window Assemblies
Torque Pressure
Reference Buffer
Sample Loading
Alignment
Titanium Rotor
Optical Detection Systems
High Vacuum
Sample Cell Components
Handling Precautions
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