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表面固定化生体分子の単一分子蛍光測定のための自動化システム
JoVE Journal
Biology
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Automated System for Single Molecule Fluorescence Measurements of Surface-immobilized Biomolecules
Please note that all translations are automatically generated.
Click here for the English version.
表面固定化生体分子の単一分子蛍光測定のための自動化システム
DOI:
10.3791/1542-v
•
10:57 min
•
November 02, 2009
•
Nicolas Di Fiori
,
Amit Meller
2
1
Physics Department
,
Boston University
,
2
Department of Biomedical Engineering
,
Boston University
Chapters
00:00
Title
01:44
Introduction
02:12
Assembling the Microfluidic Cell
05:35
Activating the Surface of the Microfluidic Cell for Molecule Immobilization
07:31
Preparing the Imaging Buffer and Scanning the Sample
10:03
Time-Resolved Single Molecule FRET Traces of DNA Molecules
10:31
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
この記事では、我々は、自動走査型共焦点顕微鏡を用いて表面に固定化された個々のDNA分子からのトレースをFRETの取得方法について説明します。
Tags
Automated System
Single Molecule Fluorescence Measurements
Surface-immobilized Biomolecules
Fluorescence Resonance Energy Transfer Microscopy
FRET Microscopy
Nucleic Acids
Proteins
Sm-FRET Measurements
Time-traces
Biomolecular Dynamics
Statistical Analyses
Automated Scanning Confocal Microscope
Microfluidic Cell
Buffer Exchange
Oxygen Content
Constant Temperature
Microfluidic Device Assembly
DNA Immobilization
Buffer Preparation
Photostability
Fluorophores
Setup Preparation
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