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Journal
/
Biology
/
Dopamine Release at Individual Presynaptic Terminals Visualized with FFNs
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Dopamine Release at Individual Presynaptic Terminals Visualized with FFNs
Dopamine Release at Individual Presynaptic Terminals Visualized with FFNs
DOI:
10.3791/1562-v
•
09:37 min
•
August 31, 2009
•
Hui Zhang
2
,
Niko G. Gubernator
4
,
Minerva Yue
,
Roland G. W. Staal
,
Eugene V. Mosharov
,
Daniela Pereira
,
Vojtech Balsanek
,
Paul A. Vadola
,
Bipasha Mukherjee
,
Robert H. Edwards
,
David Sulzer
2,6
,
Dalibor Sames
1
Departments of Neurology
,
Columbia University
,
2
Departments of Psychiatry and Pharmacology
,
Columbia University
,
3
Department of Chemistry
,
Columbia University
,
4
eMolecules, Inc.
,
5
Departments of Neurology and Physiology
,
University of California School of Medicine, San Francisco
,
6
Division of Molecular Therapeutics
,
New York Psychiatric Institute
Chapters
00:06
Title
01:15
Introduction
01:41
Preparing Acute Striatal Slices and Loading FFN511
03:59
Imaging FFN511 in the Brain Slices
05:43
FFN511 Destaining
07:03
Results of FFN511 Loading and Destaining in Brain Slices
08:44
Conclusion
Summary
Automatic Translation
English (Original)
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italiano (Italian)
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Automatic Translation
A new means to measure neurotransmission optically using fluorescent dopamine analogs.
Tags
Dopamine Release
Individual Presynaptic Terminals
FFNs
Neurotransmitter Uptake
Synaptic Vesicle Fusion
Fluorescent False Neurotransmitters
Synaptic Plasticity
Stimulus Frequency
Reserve Synaptic Vesicle Population
D2 Dopamine Receptors
Frequency-dependent Coding
Presynaptic Selection
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