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在豚鼠肠道网络，利用电压敏感染料光学记录的电活动
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JoVE Journal Biology
Optical Recording of Electrical Activity in Guinea-pig Enteric Networks using Voltage-sensitive Dyes
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在豚鼠肠道网络，利用电压敏感染料光学记录的电活动

DOI:
10.3791/1631-v

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14:23 min

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December 04, 2009

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1Department of Neuroscience,University of Pennsylvania-School of Medicine, 2Department of Physiology,University of Pennsylvania-School of Medicine

Chapters

  • 00:00Title
  • 00:59Introduction
  • 01:46Tissue Preparation
  • 05:39Preparation of Tissue Samples for Optical Recording
  • 07:10Optical Recording of Electrical Activity
  • 10:30Image Analysis
  • 12:00Representative Optical Data
  • 13:14Conclusion

Summary

Automatic Translation

Automatic Translation

该协议说明了电压敏感染料如何使豚鼠肠神经系统的神经丛，如完整的神经网络电活动的可调节分辨率的光学记录，范围从单细胞到多节后电路。

Tags

Optical RecordingElectrical ActivityGuinea-pig Enteric NetworksVoltage-sensitive DyesEnteric Nervous SystemMyenteric PlexusSubmucous PlexusReflex PathwaysGangliaSynaptic InteractionsGastrointestinal FunctionExperimental ModelMolecular EventsCellular EventsNeuronal NetworkPhysiological Outputs

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