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Chapters
- 00:00Title
- 01:15Introduction
- 03:01Plasmid DNA Constructions
- 04:04Streak Analysis of the Slow Growth Phenotype Restoration in WRN Transformed sgs1 top3 Strain
- 06:00Genetic Analysis of Hydroxyurea (HU) or Methylmethane-sulfonate (MMS) Sensitivity in WRN Transformed Yeast Strains
- 07:24Determining Cell Cycle Distribution of the WRN Transformated sgs1 top3 Strain
- 09:21Preparing Cell Lysates for Western Blot Analysis
- 11:09Effects of WRN Expression in sgs1 and top3 Yeast Cells
- 13:23Conclusion
Los estudios genéticos de la levadura puede ser empleado para investigar las funciones celulares y moleculares de los genes humanos en el metabolismo del ADN celular. Se describen los métodos para la caracterización genética de los humanos<em> WRN</em> Producto del gen defectuoso en el síndrome de envejecimiento prematuro Werner trastorno funcional en las vías de conservación con la levadura como un sistema modelo manejable.
Tags
Genetic StudiesHuman DNA Repair ProteinsYeast Model SystemMammalian SystemsModel Genetic SystemsLower EukaryotesWerner Syndrome Helicase-nuclease (WRN)Nucleic Acid MetabolismCellular PhenotypesGenetic Mutant BackgroundsCatalytic ActivitiesProtein InteractionsWRN GeneDNA PlasmidsRegulatory Plasmid ElementYeast Mutant BackgroundGenomic Stability
