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从外植体生长的首要人权支气管上皮细胞
JoVE Journal
Biology
Author Produced
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JoVE Journal Biology
Primary Human Bronchial Epithelial Cells Grown from Explants
DOI:

14:32 min

March 26, 2010

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Chapters

  • 00:00Title
  • 00:58Coating and Scratching 100mm Culture Plates
  • 04:58Bronchial Tissue Transplants
  • 05:41Passage of Human Bronchial Epithelial (HBE) Cells
  • 08:42Growing Ciliated HBE Cells on Transwells
  • 11:42Representative Results
  • 13:54Conclusion

Summary

Automatic Translation

在这里,我们描述一个人支气管气道组织的外植体,包括气 - 液界面上的区别的增长为发展初级人支气管上皮细胞的具体方法。这种方法提供了一个丰富的原代细胞源调查在人的肺部健康和疾病的气道上皮细胞的作用。

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