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含量测定扩散的信号分子能够重新调整胚胎脊髓连合轴突
JoVE Journal
Biology
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JoVE Journal Biology
Assaying the Ability of Diffusible Signaling Molecules to Reorient Embryonic Spinal Commissural Axons
DOI:

09:28 min

March 08, 2010

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Chapters

  • 00:00Title
  • 00:28Introduction
  • 01:14Preparation of Aggregates of Transfected COS Cells Using Hanging Drops
  • 02:20Preparation of Dorsal Spinal Cord Explants
  • 03:31Priming Collagen
  • 04:31Dissection of Dorsal Spinal Cord Explants and Positioning with COS Cell Aggregates in Collagen Matrix
  • 06:51Visualization of Commissural Axons By Immunohistochemistry
  • 08:03Representative Images of Explants
  • 08:41Conclusion

Summary

Automatic Translation

这种检测评估的一种信号分子的能力,这里骨形态发生蛋白7(BMP7),调整连合轴突。一个胚胎脊髓背侧的外植体是培养毗邻COS细胞分泌候选人生长因子的总和。重新调整连合轴突生长外植体内免疫组织化学显示。

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