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Chapters
- 00:00Title
- 00:57Introduction
- 01:40Sample Preparation for the Msh2-Msh6/ DNA Binding Experiment
- 03:07Instrument Preparation for the Msh-2Msh6/DNA Binding Experiment
- 05:07Msh2-Msh6/DNA Binding Experiment
- 06:27Representative Results for the Msh2-Msh6/DNA Binding Kinietics Experiment
- 07:00Sample and Instrument Preparation for the Msh2-Msh6 ATPase Experiment
- 08:30Msh2-Msh6 ATPase Experiment
- 09:40Representative Results for Msh2-Msh6 ATPase Kinetics Experiments
- 10:20Conclusion
MSH2-MSH6 ist verantwortlich für die Initiierung Reparatur der Replikation Fehler bei der DNA. Hier präsentieren wir eine transiente Kinetik Ansatz zum Verständnis, wie diese kritische Protein funktioniert. Der Bericht zeigt, stopped-flow Experimente zur Messung der gekoppelten DNA-Bindung und ATPase-Kinetik zugrunde liegenden MSH2-MSH6 Wirkmechanismus in der DNA-Reparatur.
Tags
Stopped-flow Kinetics MethodsMechanism Of ActionDNA Repair ProteinTransient Kinetic AnalysisBiological MacromoleculesActive Site ConcentrationsIntrinsic Rate ConstantsMacromolecular FunctionEnzymesPre-steady State MeasurementsReaction PathwaySteady State MeasurementsCatalytic EfficiencyCatalytic SpecificityProtein-protein InteractionsProtein-ligand InteractionsRate-limiting Conformational ChangesMillisecond TimescaleStopped-flow MethodChemical-quench Flow MethodsOptical SignalFluorescenceSubstrate BindingProduct ReleaseCatalytic TurnoverMsh2-Msh6 ProteinEukaryotic DNA Repair ProteinBase-pair MismatchesInsertion/deletion Loops In DNAMismatch Repair (MMR)Genomic Integrity
