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有丝分裂的siRNA转染后的时间推移成像
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Time-lapse Imaging of Mitosis After siRNA Transfection
Please note that all translations are automatically generated.
Click here for the English version.
有丝分裂的siRNA转染后的时间推移成像
DOI:
10.3791/1878-v
•
08:21 min
•
June 06, 2010
•
Douglas R. Mackay
,
Katharine S. Ullman
,
Christopher K. Rodesch
1
Department of Oncological Sciences, Huntsman Cancer Institute
,
University of Utah
,
2
Fluorescence Microscopy Core Facility
,
University of Utah
Chapters
00:00
Title
00:45
Introduction
01:11
siRNA Transfection and Cell Preparation
02:18
Microscope Setup and Image Acquisition
05:22
Image Processing and Analysis
07:21
Effect of Knockdown of Nucleoporin Nup153
07:48
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里,我们描述了一个基本的协议,以图像和量化现场后,转染哺乳动物组织培养细胞的有丝分裂的时机。
Tags
Time-lapse Imaging
Mitosis
SiRNA Transfection
Cellular Organization
Chromosome Dynamics
Fluorescence Microscopy
GFP-tagged Proteins
RNAi-based Depletion
Mitotic Regulatory Protein
Automated Fluorescence Microscope
Image Sequences
Still-image Montages
Movies
Quantification Of Mitotic Stages
MCherry-tagged Histone H2B
Time-lapse Experiment Design
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