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N -连接糖链的糖蛋白在哺乳动物细胞中的脉冲大通分析
JoVE Journal
Biology
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JoVE Journal Biology
Pulse-chase Analysis of N-linked Sugar Chains from Glycoproteins in Mammalian Cells
DOI:

10:17 min

April 27, 2010

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Chapters

  • 00:00Title
  • 00:48Introduction
  • 01:28Pulse-chase Labeling and Protein Extraction
  • 04:34Immunoprecipitation (IP)
  • 05:38Deglycosylation and Preparing the Samples for HPLCE Analysis
  • 08:05Analysis of N-glycans from NIH 3T3 Glycoproteins Representative Results
  • 09:24Conclusion

Summary

Automatic Translation

我们描述了一种通过改变早期的生活后,其在哺乳动物细胞中合成的糖蛋白的N -连接聚糖分析方法。这是通过分析代谢标记聚糖,从糖蛋白和高效液相色谱法检查酶的释放脉冲追逐。

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