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Journal
/
Biology
/
微图案的表面上的蛋白质-蛋白质相互作用
的
体内检测
JoVE Journal
Biology
Author Produced
This content is Free Access.
JoVE Journal
Biology
In-vivo
Detection of Protein-protein Interactions on Micro-patterned Surfaces
Please note that all translations are automatically generated.
Click here for the English version.
微图案的表面上的蛋白质-蛋白质相互作用
的
体内检测
DOI:
10.3791/1969-v
•
07:42 min
•
March 19, 2010
•
Julian Weghuber
,
Stefan Sunzenauer
,
Mario Brameshuber
,
Birgit Plochberger
,
Clemens Hesch
,
Gerhard J. Schutz
1
Institute of Biophysics
,
Johannes Kepler Universitat Linz
Chapters
00:00
Title
00:07
Introduction
02:17
μ-contact Printing
04:15
Microscopy
05:50
Data Analysis
06:27
Representative Results
06:56
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该视频显示与后续分析使用微图案的表面蛋白质相互作用的实验。该方法提供了检测在活细胞内蛋白质相互作用的可能性,并结合在一起的可能性高吞吐能力,提取定量信息。
Tags
In-vivo Detection
Protein-protein Interactions
Micro-patterned Surfaces
Interaction Network
Molecules
Cell Function
Metabolism
Methodological Options
Molecular Interactions
Quantitative
High Throughput
High-end Quantitative Approaches
Protein-protein Interactions System
Fluorophore-labeled Protein
Prey And Bait Interactions
Micropatterned Surfaces
Antibodies
Bait Exoplasmic Domain
Fluorescent Prey Redistribution
High Sensitivity
Weak Interactions
Screening Tool
Article
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