Journal
/
/
闪存冻结和Cryosectioning E12.5小鼠脑
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Flash Freezing and Cryosectioning E12.5 Mouse Brain
DOI:

28:32 min

May 28, 2007

,

Chapters

  • 00:06Introduction
  • 00:18Tools and reagents
  • 01:23Preparation of the cryomold
  • 04:45Orienting tissue
  • 07:52Flash freezing
  • 09:39Using cryostat
  • 11:33Preparation and mounting tissue for sectioning
  • 19:33Sectioning
  • 27:36Conclusion

Summary

Automatic Translation

这个视频展示了闪光冻结和从小鼠胚胎脑组织切片技术。使用低温恒温器的有用提示,其中包括排除故障的方法,可以用来切割,以确保由此产生的组织部分无裂缝和其他扭曲。

Related Videos

Read Article