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Biology
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後期ステージショウジョウバエ蛹の脳からの初代神経培養
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JoVE Journal
Biology
Primary Neuronal Cultures from the Brains of Late Stage Drosophila Pupae
Please note that all translations are automatically generated.
Click here for the English version.
後期ステージショウジョウバエ蛹の脳からの初代神経培養
DOI:
10.3791/200-v
•
15:12 min
•
May 28, 2007
•
Beatriz Sicaeros
,
Jorge M. Campusano
,
Diane K. O’Dowd
1
Department of Development and Cell Biology, Department of Anatomy and Neurobiology
,
University of California, Irvine (UCI)
Chapters
00:19
Introduction
01:14
Preparation
01:54
Collection of pupae
02:36
Isolating pupae head
04:13
Isolating brain
05:57
Processing isolated brain
07:04
Washing off enzyme solution
07:58
Trituration and plating
12:23
Imaging calcium flux in pupal neurons
13:26
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
このビデオでは、後期ショウジョウバエ蛹の脳からの初代神経培養の準備を示しています。ライブ文化の景色は、フラ-2を使用してカルシウム濃度の神経突起伸長とイメージングを示す。
Tags
Primary Neuronal Cultures
Late Stage Drosophila Pupae
Brain Removal
Papain Incubation
Serum-free Growth Medium
Mechanical Dissociation
Axons
Dendrites
Regeneration
Live Cultures
GAL4 Lines
Fluorescent Markers
Whole Cell Recordings
Cholinergic Synapses
GABAergic Synapses
Calcium Dynamics
Fura-2 Dye
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Pharmacological Tools
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