A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:19Introduction
- 01:14Preparation
- 01:54Collection of pupae
- 02:36Isolating pupae head
- 04:13Isolating brain
- 05:57Processing isolated brain
- 07:04Washing off enzyme solution
- 07:58Trituration and plating
- 12:23Imaging calcium flux in pupal neurons
- 13:26Conclusion
Tags
Primary Neuronal CulturesLate Stage Drosophila PupaeBrain RemovalPapain IncubationSerum-free Growth MediumMechanical DissociationAxonsDendritesRegenerationLive CulturesGAL4 LinesFluorescent MarkersWhole Cell RecordingsCholinergic SynapsesGABAergic SynapsesCalcium DynamicsFura-2 DyeGenetic ToolsPharmacological Tools
