JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Biology
/
在哺乳动物细胞中的DNA双链断裂(DSB)的维修分析
JoVE Journal
Biology
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Biology
Analysis of DNA Double-strand Break (DSB) Repair in Mammalian Cells
Please note that all translations are automatically generated.
Click here for the English version.
在哺乳动物细胞中的DNA双链断裂(DSB)的维修分析
DOI:
10.3791/2002-v
•
13:10 min
•
September 08, 2010
•
Andrei Seluanov
,
Zhiyong Mao
,
Vera Gorbunova
1
Department of Biology
,
University of Rochester
Chapters
00:00
Title
01:13
Introduction
02:22
The Reporter Constructs
05:02
Integration of the Reporter Constructs and Induction of DSBs
09:18
Analysis of the GFP+ and DsRed+ Cells by Flow Cytometry
11:34
Representative FACS Results of the DSB Repair Assay
12:31
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
本文介绍了基于GFP的荧光<em>在体内</em>分析,分别量化同源重组和非同源末端在哺乳动物细胞中加入。
Tags
DNA Double-strand Break
Repair
Mammalian Cells
Nonhomologous End Joining
NHEJ
Homologous Recombination
HR
Perturbations
Premature Aging
Tumorigenesis
Quantitative Measurement
Fluorescent Reporter Constructs
GFP Gene
I-SceI Endonuclease
Induction Of DSBs
GFP Positive Cells
Flow Cytometry
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
使用哺乳动物骨骼肌的DNA转染
在体内
电穿孔
游离成年哺乳动物元DNA的细胞核内内微量注射
N -连接糖链的糖蛋白在哺乳动物细胞中的脉冲大通分析
异源表达在哺乳动物细胞离子通道的突变和功能分析
雪旺的星形胶质细胞使用相互作用分析<em在体外检测
在哺乳动物细胞通过显微注射mRNA的核出口动力学分析
在哺乳动物细胞中细胞周期位置分析
单工程的RNA转录物的活细胞实时成像采用比例式双分子信标
在联合电子能谱成像miniSOG标记的DNA修复蛋白可视化(ESI)
考试蛋白在哺乳动物细胞中绑定到新生的DNA使用的BrdU芯片插槽西方技术
Read Article