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Análise de DNA dupla fita-Break Repair (DSB) em células de mamíferos
JoVE Journal
Biology
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JoVE Journal Biology
Analysis of DNA Double-strand Break (DSB) Repair in Mammalian Cells
DOI:

13:10 min

September 08, 2010

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Chapters

  • 00:00Title
  • 01:13Introduction
  • 02:22The Reporter Constructs
  • 05:02Integration of the Reporter Constructs and Induction of DSBs
  • 09:18Analysis of the GFP+ and DsRed+ Cells by Flow Cytometry
  • 11:34Representative FACS Results of the DSB Repair Assay
  • 12:31Conclusion

Summary

Automatic Translation

Este artigo descreve GFP baseada em fluorescência<em> In vivo</em> Ensaios que quantificar separadamente recombinação homóloga e nonhomologous final juntar em células de mamíferos.

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