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Biology
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哺乳動物細胞におけるDNA二重鎖切断(DSB)修復の解析
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JoVE Journal
Biology
Analysis of DNA Double-strand Break (DSB) Repair in Mammalian Cells
Please note that all translations are automatically generated.
Click here for the English version.
哺乳動物細胞におけるDNA二重鎖切断(DSB)修復の解析
DOI:
10.3791/2002-v
•
13:10 min
•
September 08, 2010
•
Andrei Seluanov
,
Zhiyong Mao
,
Vera Gorbunova
1
Department of Biology
,
University of Rochester
Chapters
00:00
Title
01:13
Introduction
02:22
The Reporter Constructs
05:02
Integration of the Reporter Constructs and Induction of DSBs
09:18
Analysis of the GFP+ and DsRed+ Cells by Flow Cytometry
11:34
Representative FACS Results of the DSB Repair Assay
12:31
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
この記事では、GFPベースの蛍光を示して<em> in vivoで</em>別々に相同組換えを定量化し、非相同哺乳類細胞に結合する終了アッセイ。
Tags
DNA Double-strand Break
Repair
Mammalian Cells
Nonhomologous End Joining
NHEJ
Homologous Recombination
HR
Perturbations
Premature Aging
Tumorigenesis
Quantitative Measurement
Fluorescent Reporter Constructs
GFP Gene
I-SceI Endonuclease
Induction Of DSBs
GFP Positive Cells
Flow Cytometry
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