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ソフト筋骨格組織から幹細胞を単離する
JoVE Journal
Biology
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JoVE Journal
Biology
Isolating Stem Cells from Soft Musculoskeletal Tissues
Please note that all translations are automatically generated.
Click here for the English version.
ソフト筋骨格組織から幹細胞を単離する
DOI:
10.3791/2011-v
•
07:49 min
•
July 05, 2010
•
Yong Li
2,3,4
,
Haiying Pan
,
Johnny Huard
2,3,4,5
1
Stem Cell Research Center, Childrens Hospital of Pittsburgh of UPMC
,
2
Department of Bioengineering
,
University of Pittsburgh
,
3
Department of Orthopedic Surgery
,
University of Pittsburgh
,
4
Department of Pathology
,
University of Pittsburgh
,
5
Department of Molecular Genetics & Biochemistry
,
University of Pittsburgh
Chapters
00:00
Title
00:49
Introduction
01:52
Mechanical Dissociation and Enzymatic Digestion of Tissues
04:27
Preplating to Isolate Different Cell Populations Based on Cell Adhesion Rates
06:14
Identification of Different Cell Populations
06:59
Conclusion
Summary
Automatic Translation
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Automatic Translation
フラスコに細胞の付着の速度に基づいて、筋骨格系の軟部組織から成体幹細胞を単離する。
Tags
Stem Cells
Regenerative Medicine
Adult Stem Cells
Multi-lineage Cell Differentiation
Self-renewal Ability
Autologous Cell Transplantation Therapies
Preplate Technique
Enzymatically Dissociated
Collagen Coated Flasks
Supernatant
Serial Transfers
Non-invasive Procedures
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