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Isolera stamceller från Soft Muskuloskeletala vävnader
JoVE Journal
Biology
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JoVE Journal
Biology
Isolating Stem Cells from Soft Musculoskeletal Tissues
Please note that all translations are automatically generated.
Click here for the English version.
Isolera stamceller från Soft Muskuloskeletala vävnader
DOI:
10.3791/2011-v
•
07:49 min
•
July 05, 2010
•
Yong Li
2,3,4
,
Haiying Pan
,
Johnny Huard
2,3,4,5
1
Stem Cell Research Center, Childrens Hospital of Pittsburgh of UPMC
,
2
Department of Bioengineering
,
University of Pittsburgh
,
3
Department of Orthopedic Surgery
,
University of Pittsburgh
,
4
Department of Pathology
,
University of Pittsburgh
,
5
Department of Molecular Genetics & Biochemistry
,
University of Pittsburgh
Chapters
00:00
Title
00:49
Introduction
01:52
Mechanical Dissociation and Enzymatic Digestion of Tissues
04:27
Preplating to Isolate Different Cell Populations Based on Cell Adhesion Rates
06:14
Identification of Different Cell Populations
06:59
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
Isolera stamceller från rörelseorganens mjukdelar baserat på cellens anslutning hastighet till kolv.
Tags
Stem Cells
Regenerative Medicine
Adult Stem Cells
Multi-lineage Cell Differentiation
Self-renewal Ability
Autologous Cell Transplantation Therapies
Preplate Technique
Enzymatically Dissociated
Collagen Coated Flasks
Supernatant
Serial Transfers
Non-invasive Procedures
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