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比较在体内 GP96佐剂研究中的青蛙非洲爪蟾</em
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Comparative in vivo Study of gp96 Adjuvanticity in the Frog Xenopus laevis
DOI:

11:20 min

September 16, 2010

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Chapters

  • 00:00Title
  • 01:24Introduction
  • 02:12Elicitation and Harvest of Peritoneal Leukocytes (PLs)
  • 03:43Pulsing and Adoptive Transfer of LG-6 PLs into Naive LG-6 Recipients
  • 05:20Skin Graft Assay
  • 07:14Tumor Transplantation Assay
  • 07:56Collecting Blood Leukocytes
  • 08:58Skin Graft and Tumor Transplantation Results
  • 10:34Conclusion

Summary

Automatic Translation

青蛙<em>非洲爪蟾</em>提供了一个有吸引力的替代非哺乳动物模型探讨热休克蛋白等作为GP96的能力,促进抗原特异性CD8 T细胞反应。我们目前的方法来研究<em>在体内</em> GP96促进皮肤和肿瘤抗原的交叉呈现。

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