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Comparative In vivo Etude de gp96 adjuvanticité de la grenouille Xenopus laevis</em
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Chapters
- 00:00Title
- 01:24Introduction
- 02:12Elicitation and Harvest of Peritoneal Leukocytes (PLs)
- 03:43Pulsing and Adoptive Transfer of LG-6 PLs into Naive LG-6 Recipients
- 05:20Skin Graft Assay
- 07:14Tumor Transplantation Assay
- 07:56Collecting Blood Leukocytes
- 08:58Skin Graft and Tumor Transplantation Results
- 10:34Conclusion
La grenouille<em> Xenopus laevis</em> Fournit une intéressante alternative non-mammifère modèle pour explorer la capacité de la protéine de choc thermique tels que gp96 à promouvoir spécifiques de l'antigène des cellules T CD8. Nous présentons des méthodes pour étudier<em> In vivo</emFacilitation> des présentation croisée d'antigènes tumoraux par la peau et gp96.
Tags
Gp96 AdjuvanticityComparative In Vivo StudyFrog Xenopus LaevisHeat Shock ProteinsChaperoned AntigensInnate And Adaptive T Cell ResponsesXenopus Skin Graft RejectionMHC Class Ia Restricted T Cell ResponsesTumors Down-regulating Class Ia MoleculesAdoptive Cell Transfer AssayPeritoneal LeukocytesAntigen Presenting Cells (APCs)CD8 T Cell ResponsesMinor Histocompatibility Skin AntigensXenopus Thymic Tumor 15/0MethodologyElicitationPulsingAdoptive TransferSkin Graft Transplantation AssayTumor Transplantation AssayHarvesting Peripheral Blood LeukocytesFlow CytometryProliferation Assays
