/
/
Comparativo In vivo Estudo da gp96 adjuvante no Sapo Xenopus laevis</em
A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:00Title
- 01:24Introduction
- 02:12Elicitation and Harvest of Peritoneal Leukocytes (PLs)
- 03:43Pulsing and Adoptive Transfer of LG-6 PLs into Naive LG-6 Recipients
- 05:20Skin Graft Assay
- 07:14Tumor Transplantation Assay
- 07:56Collecting Blood Leukocytes
- 08:58Skin Graft and Tumor Transplantation Results
- 10:34Conclusion
O sapo<em> Xenopus laevis</em> Oferece um atraente modelo de não-mamíferos alternativa para explorar a capacidade da proteína de choque térmico, tais como para promover a gp96 antígeno-específica de células T CD8 respostas. Nós apresentamos métodos para o estudo<em> In vivo</em> Facilitação de apresentação cruzada de antígenos de tumor de pele e gp96.
Tags
Gp96 AdjuvanticityComparative In Vivo StudyFrog Xenopus LaevisHeat Shock ProteinsChaperoned AntigensInnate And Adaptive T Cell ResponsesXenopus Skin Graft RejectionMHC Class Ia Restricted T Cell ResponsesTumors Down-regulating Class Ia MoleculesAdoptive Cell Transfer AssayPeritoneal LeukocytesAntigen Presenting Cells (APCs)CD8 T Cell ResponsesMinor Histocompatibility Skin AntigensXenopus Thymic Tumor 15/0MethodologyElicitationPulsingAdoptive TransferSkin Graft Transplantation AssayTumor Transplantation AssayHarvesting Peripheral Blood LeukocytesFlow CytometryProliferation Assays
