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Comparativo In vivo Studio di gp96 Adjuvanticity nella rana Xenopus laevis</em
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Comparative in vivo Study of gp96 Adjuvanticity in the Frog Xenopus laevis
DOI:

11:20 min

September 16, 2010

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Chapters

  • 00:00Title
  • 01:24Introduction
  • 02:12Elicitation and Harvest of Peritoneal Leukocytes (PLs)
  • 03:43Pulsing and Adoptive Transfer of LG-6 PLs into Naive LG-6 Recipients
  • 05:20Skin Graft Assay
  • 07:14Tumor Transplantation Assay
  • 07:56Collecting Blood Leukocytes
  • 08:58Skin Graft and Tumor Transplantation Results
  • 10:34Conclusion

Summary

Automatic Translation

La rana<em> Xenopus laevis</em> Offre una interessante alternativa non-mammiferi modello per esplorare la possibilità di proteine ​​da shock termico come gp96 per promuovere antigene-specifiche risposte delle cellule T CD8. Vi presentiamo i metodi per lo studio<em> In vivo</emFacilitazione> di cross-presentazione di antigeni tumorali della pelle e da gp96.

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