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一个慢性双光子薄颅骨窗口技术在体内 Neuroinflammation模型小鼠小胶质细胞成像
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
A Thin-skull Window Technique for Chronic Two-photon In vivo Imaging of Murine Microglia in Models of Neuroinflammation
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一个慢性双光子薄颅骨窗口技术在体内 Neuroinflammation模型小鼠小胶质细胞成像

DOI:
10.3791/2059-v

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12:07 min

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September 19, 2010

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, , , ,
1Center for Neural Development and Disease, Department of Neurology, Child Neurology Division,University of Rochester, 2Department of Neurobiology and Anatomy,University of Rochester

Chapters

  • 00:00Title
  • 01:14Introduction
  • 01:47Preparing the Animal for Imaging
  • 04:46Preparing the Thinned Skull Cortical Window
  • 07:00Two-photon Imaging
  • 08:48Injection of the HIV-1 Neurotoxin, Tat
  • 11:37Conclusion

Summary

Automatic Translation

Automatic Translation

我们描述了一个反复可视化小鼠小胶质细胞和单核细胞循环的方法<em>在体内</em>超过几小时，几天或几周内，采用经颅双光子显微镜。我们将演示如何编写一个薄的头骨的窗口，让静态的小胶质细胞，激活，可以通过相邻的立体定向注射HIV - 1调控蛋白达间歇观察。

Tags

Thin-skull Window TechniqueChronic Two-photon In Vivo ImagingMurine MicrogliaNeuroinflammationOpen-skull TechniqueThinned-skull ApproachMicroglial ActivationChronic ImagingImaging SessionsTwo-photon MicroscopyStable Cortical WindowGlass CoverslipTranslucentImmunologically InertCX3CR1 GFP/+ MicePial SurfaceStereotactic Brain Injury

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