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海马脑片的器官型检查神经元损伤的文化模式
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Neuroscience
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JoVE Journal Neuroscience
The Organotypic Hippocampal Slice Culture Model for Examining Neuronal Injury
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海马脑片的器官型检查神经元损伤的文化模式

DOI:
10.3791/2106-v

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06:33 min

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October 28, 2010

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1Department of Neurology and Neurological Sciences,Stanford University School of Medicine

Chapters

  • 00:00Title
  • 01:39Preparing Slice Cultures
  • 03:51Inducing and Quantifying Hippocampal Neuronal Injury in Slice Culture
  • 05:43Representative Images
  • 06:13Conclusion

Summary

Automatic Translation

Automatic Translation

organoptypic海马脑片培养模型是一个<em>在体外</em>模型来研究各种范式的神经元损伤。在这篇文章中，我们描述了片文化和量化神经元损伤的方法。

Tags

Organotypic Hippocampal Slice CultureNeuronal InjuryIn Vitro MethodHippocampusNeuronal ArchitectureCompositionOrganotypic Culture SystemAstrocytic EffectsMicroglial EffectsEx Vivo PreparationBlood Flow EffectsPeripheral Inflammatory CellsExcitotoxic InjuryHypoxic InjuryPyramidal NeuronsInflammatory ResponseMethods For Generating Hippocampal Slice CulturesToxic StimuliAssaying And Quantifying Neuronal Death

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