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单细胞转染鸡胚
JoVE Journal
Biology
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JoVE Journal Biology
Single Cell Transfection in Chick Embryos
DOI:

08:46 min

September 25, 2010

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Chapters

  • 00:00Title
  • 00:57Introduction
  • 01:21Micropipette, DNA, and Embryo Preparation
  • 04:15Loading Micropipettes and Microinjection
  • 06:49Representative Injection Results
  • 08:18Conclusion

Summary

Automatic Translation

使用精尖微量注入鸡体节或神经管的子域的质粒DNA。质粒的浓度调整,以产生单一的转染细胞。然后,我们允许的细胞发育成无性系种群。

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