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Immunology and Infection
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评估气孔反应活细菌细胞,采用全叶成像
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal
Immunology and Infection
Assessing Stomatal Response to Live Bacterial Cells using Whole Leaf Imaging
Please note that all translations are automatically generated.
Click here for the English version.
评估气孔反应活细菌细胞,采用全叶成像
DOI:
10.3791/2185-v
•
07:03 min
•
October 02, 2010
•
Reejana Chitrakar
,
Maeli Melotto
1
Biology
,
University of Texas at Arlington
Chapters
00:00
Title
00:50
Introduction
01:08
Growing Plants and Preparing Bacteria
02:58
Leaf Staining and Incubation with Bacteria
04:34
Microscopy, Measurement, and Data Analysis
05:21
Representative Images
06:43
Conclusion
Summary
Automatic Translation
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Automatic Translation
我们已经开发出一种简单,重复性的协议来访问气孔反应活的细菌。这种方法最大限度地减少以前相比,使用表皮换肤叶伤人及操纵报告。
Tags
Stomatal Response
Live Bacterial Cells
Whole Leaf Imaging
Guard Cells
Stomatal Pore
Gas Exchange
Light Intensity
Carbon Dioxide Concentration
Relative Humidity
Pathogen Entry
Disease Development
Arabidopsis Plants
Plant Innate Immunity
Epidermal Peels
Environmental Conditions
MES Buffer
Electrophysiological Experiments
Bacterial Suspension
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